[On a glycolipopeptide from chloroplasts and its serological properties].

نویسنده

  • D Hiedemann-van Wyk
چکیده

The methanol-ether extract of chloroplasts contains an ether-insoluble part which after purification was classified as a glycolipopeptide. This fraction can be precipitated by the antisera to the lamellar system of chloroplasts. By immunisation of rabbits with this compound, a glycolipopeptideantiserum was obtained. This antiserum precipitates the glycolipopeptide. There was only one precipitation line in the diffusion test. This antiserum agglutinates stroma-freed chloroplasts and precipitates the ultrasonic supernatant and the structural protein of stroma-freed chloroplasts. It must be concluded from the results of the agglutination inhibition experiments, that the reactive groups of the glycolipopeptide, in regard to the lamellar system antiserum, are the same as those of the structural protein and the supernatant of the sonicated lamellar system. The annulment of the immunreaction after periodate treatment points to the role of carbohydrates as the determinant groups.

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عنوان ژورنال:
  • Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete

دوره 26 10  شماره 

صفحات  -

تاریخ انتشار 1971